Using high-throughput technologies, you can identify long lists of candidate genes that differ between two experimental conditions. In order to interpret these gene lists and to discover fundamental properties like gene function and disease relevance, you need to use the annotation linked to a given gene or protein sequence.

The goals of this course and the practical exercises that follow are to give some basic theoretical and practical knowledge on protein sequence databases with a focus on UniProtKB, on Gene Ontology, on the different manual and automated annotation pipelines (such as HAMAP) and, in particular, on the optimum use of UniProt.