By modeling genome-wide gene expression and chromatin state data in terms of computationally predicted binding sites, Motif Activity Response Analysis (MARA) allows automatic inference of the key regulators, their targets, and their interactions from high-throughput data in any system. In recent years MARA has been completely automated into an integrated system (ISMARA) webserver that allows any researcher to upload their data and obtain comprehensive predictions of key regulatory network structure in their data. The ISMARA system is quite sophisticated and provides users a large number of interactive possibilities to explore predictions and to generate new analyses of the data and even many experienced users are only aware of a fraction of the possibilities that the system provides. We here propose to provide an in-depth interactive workshop of the ISMARA system.