Topic outline

  • General

    De Novo Transcriptome Assembly - Trinity

    Bern, 10-11 October 2016

    University of Bern, Hochschulstrasse 4, room 331 3.OG/West

    This page is addressed to registered participants. To access event description, exact location and application form, please click here.

    For any assistance, please contact training@sib.swiss.

    • Programme

      The course will run every day from 9h to 12h30 and then from 13h30 to 17h, and will include two coffee breaks per day.

      Day 1:

      Morning:  Intro to RNA-Seq and transcriptome analysis

      • lecture on intro to rna-seq and the Trinity algorithm for transcript reconstruction
      • practical:¬†evaluation of NGS read quality
      • practical: in silico normalization of reads
      • practical: trinity assembly

      Afternoon: Expression Quantitation and Assembly Quality Assessment

      • lecture on expression quantitation
      • practical: using RSEM and kallisto for expression quantitation
      • practical: evaluating transcript assembly quality

      Day 2:

      Morning: Differential expression analysis

      • lecture on differential expression analysis and the importance of statistics
      • practical: using edgeR to identify DE transcripts and genes
      • practical: transcript clustering

      Afternoon: Transcript functional annotation and DE cluster functional enrichment studies

      • lecture on transcript functional annotation
      • practical: using Trinotate to functionally annotate transcripts and genes
      • practical: using goseq to identify functional enrichments of DE clusters