Topic outline

  • General

    NGS - Quality control, Alignment, Visualisation

    09-10 February 2017

    Bern, Hochschulstrasse 4 - Seminarraum Nr. 331/3. OG West, University of Bern

    This page is addressed to registered participants. To access course description and application form, please click here.

    This course will be taught by Walid Gharib, for any assistance, please contact training@sib.swiss.

  • Programme

    Thursday 09 February

    Introduction to technologies and applications, NCBI E-Utilities and sequencing data retrieval  

    9:15 - 10:30 - Introduction to technologies and applications (1)

    • Roche 454 - Pyrosequencing
    • llumina - sequencing by synthesis

    10:30 - 11:00 - Coffee Break

    11:30 - 12:15 - Introduction to technologies and applications (2)

    • Pacific Biosciences - SMRT Sequencing
    • Ion Torrent Sequencing - Semiconductor sequencing

    12:15 - 13:30 - Lunch Break

    13:30 - 13:45 - NCBI: E-utilities using Unix command line
    13:45 - 15:00 - E-utilities usage - Practicals

    15:00 - 15:30 - Coffee break

    15:30 - 15:45 - Sequencing archives, SRA, ENA and DDBJ
    15h45 - 17:00 - Fetching sequencing data - Practicals (Combining E-utilities and SRA tools)

     

    Friday 10 February

    File Formats, quality assessment, cutting/trimming/filtering and sequence alignement

    9:00 - 10:00: File formats and Quality controls
    9:45 - 10:30: Interpretation of a Fastqc report and acting upon for cutting/trimming reads

    10:30 - 11:00: Coffee break

    11:00 - 12:15: Quality control - Practicals

                            - Trimming/filtering quality control - Practicals

    12:15 - 13:30 - Lunch Break

    13:30 - 15:30 -  Alignment to a reference genome - Small intro and practicals
    16:00 - 17:00 -  Sorting, Indexing the alignment and quick visualization using IGV genome viewer

    • Prerequisites

      Participants should install "The Integrative Genome Viewer (IGV)" on their respective machines.

      Basic understanding of working with command line tools on Linux or Windows-based operating systems is required. If you do not feel comfortable with UNIX commands, please take our UNIX fundamentals e-learning module.